the aid library

Protocol Genomic DNA extraction from freshwater snail tissues

Bonner, K.; S. Bollmann, C. Adema, Danish Bilharziosis Laboratoryeschi (2024) CC

This protocol is to help researchers working with freshwater gastropods related to the transmission of schistosomes, and particularly those looking at extracting high quality intact DNA for next generation sequencing purposes. This protocol offers a ... more

Detection of Schistosoma mansoni DNA using polymerase chain reaction from serum and dried blood spot card samples of an adult population in North-western Tanzania

Fuss, A.; H. D. Mazigo and A. Mueller (2021) CC

Infect Dis Poverty (2021) 10:15. Real-time polymerase chain reaction (PCR) is a sensitive and specific method for diagnosing schistoso- miasis. However, this method should be performed in a laboratory, usually located distant from the sample collection site. Therefore, it is important to have fas ... more

Do vaccines alter your DNA?

Science in Motion (2021) CC

Introduction of DNA-based identification and typing methods to public health practitioners for epidemiological investigation of cholera outbreaks

Global Task Force on Cholera Control (GTFCC) World Health Organisation (WHO) (2017) C_WHO

The GTFCC Laboratory Support for Public Health Surveillance document provides guidelines on using DNA-based molecular techniques for identifying and monitoring Vibrio cholerae strains in cholera outbreaks. It highlights the importance of genetic seq ... more

Rapid Extraction Method of Mycobacterium ulcerans DNA from Clinical Samples of Suspected Buruli Ulcer Patients

Frimpong, M.; H. Senanu Ahor; S.A. Sakyi, et al. (2019) CC

Diagnostics 2019, 9(4), 204; https://doi.org/10.3390/diagnostics9040204

Pathology of Schistosomiasis

Roy, S. (2022) CC

DNA studies of Egyptian mummies shows evidence of the existence of Schistosomiasis about 5000 years ago. Schistosomiasis is increasing in prevalence, affecting nearly 10% of the world’s population and ranking second only to malaria as a cause of ... more

What are nucleic acid vaccines and how could they be turned against COVID-19?

GAVI (2021) CC

All vaccines work by exposing the body to molecules from the target pathogen to trigger an immune response – but the method of exposure varies. Here’s how DNA and RNA vaccines work.

Mpox Technical Factsheet

Africa CDC (2024) CC

Mpox is a zoonotic disease caused by a double-stranded DNA virus that belongs to the Orthopoxvirus genus of the Poxviridae family. The disease presents with symptoms similar to smallpox but with a lesser severity. It was first discovered in 1958 whe ... more

Monkeypox Technical Factsheet

African Union; Africa CDC Centres for Disease Control and Prevention (2022) C1

Monkeypox is a rare viral zoonotic disease caused by a double stranded DNA virus that belongs to the Orthopoxvirus genus of the Poxviridae family.

Guidelines for the prevention, diagnosis, care and treatment for people with chronic hepatitis B infection (March 2024)

World Health Organization WHO (2024) C_WHO

Hepatitis B (HBV) infection is a major public health problem and cause of chronic liver disease. The 2024 HBV guidelines provide updated evidence-informed recommendations on key priority topics. These include expanded and simplified treatment criteria for adults but now also for adolescents; expa ... more

Evaluation of serum-based real-time PCR to detect Schistosoma mansoni infection before and after treatment

Fuss, A.; H. D. Mazigo and A. Mueller (2020) CC

Infectious Diseases of Poverty (2020) 9:74 To detect acute schistosomiasis, low-intensity infections, or to verify the success of treatment with praziquantel, highly sensitive test methods are required. The aim of this study was therefore to demonstrate the performance of Schistosoma mansoni spec ... more

Mpox Testing Strategy - November 2024

Africa Center for Disease Control and Prevention CDC (2024) CC

Mpox can spread in humans through close contact, usually skin-to-skin contact, including sexual contact, with an infected person or animal, as well as with materials contaminated with the virus such as clothing, beddings and towels, and respiratory droplets in prolonged face to face contact. People ... more

Comparison of sensitivity and specificity of three diagnostic tests to detect Schistosoma mansoni infections in school children in Mwanza Region, Tanzania

Fuss, A.; H.D. Mazigo; D. Tappe, C.Kasang, A. Mueller (2018) CC

PLoS ONE 13(8): e0202499. https://doi.org/10.1371/journal.pone.0202499 This was a school-based cross-sectional study conducted in 2015 among 305 school children aged 7–16 years from two primary schools located in Ilemela and Magu Districts, north-western Tanzania. Single stool and urine samples w ... more

Laboratory testing for the monkeypox virus
recommended

World Health Organization (WHO) (2022) C_WHO

Any individual that meets the suspected case definition of monkeypox should be offered testing in appropriately equipped laboratories by staff trained in the relevant technical and safety procedures. Confirmation of monkeypox virus infection is based on nucleic acid amplification testing (NAAT), usi ... more

Comparison of the Influence of Different Nucleic Acid Extraction Assays on the Sensitivity of Trypanosoma cruzi-Specific Real-Time PCR

Kann, S.; Zabala-Monterroza, W.; García, C. Multidisciplinary Digital Publishing Institute MDPI (2022) CC

For the molecular diagnosis of Chagas disease by real-time PCR (polymerase chain reaction), optimization of diagnostic accuracy is desirable. The detection limit of real-time PCR assays for the diagnosis of Trypanosoma cruzi in human serum is affected by various influences including the choice of th ... more

Management of Dead Bodies After Disasters: A Field Manual For First Responders. (Arabic Version)
recommended

Cordner, S.; R. Coninx; J.-J. Kim; et al. PAHOWorld Health Organization; ICRC; IFRC (2016) C_WHO

Second edition. This revised edition incorporates experience gained in recent catastrophes, such as the 2013 Typhoon Haiyan in the Philippines, the 2014/15 Ebola epidemic in West Africa and the 2015 earthquake in Nepal. It also contains a number of annexes, which cover such topics as handling the bo ... more

The Top COVID-19 Vaccine Myths Spreading Online

Gregory, J. NewsGuard (2021) CC

Scientists and researchers managed to produce vaccines to protect against COVID-19. Vaccine candidates have recently been approved in some countries and are in the approval process in others, yet misinformation about the safety and effects of any future vaccine is already threatening its rollout. In ... more

Mythes & COVID-19 Vaccins
recommended

Mauder, S.; and P. Reijer (2021) CC

MEDBOX Dossier no. 16. 28 Sept 2021. De nombreuses études ont montré l'efficacité de la vaccination contre le COVID-19 et le fait qu'elle protège contre les maladies graves. maladie grave. Un taux de vaccination élevé est nécessaire pour combattre la pandémie dans le monde entier. En raiso ... more

Myths & COVID-19 Vaccinations
recommended

Mauder, S.; and P. Reijer (2021) CC

Issue Brief no. 16. 20 Sept 2021. Many studies have shown the effectiveness of vaccination against COVID-19 and that it protects against severe illness. A high vaccination rate is needed to combat the pandemic worldwide. Due to misinformation and myths, there is still a great hesitancy to vaccina ... more