American trypanosomiasis, commonly called Chagas disease (CD), was prevalent once in rural areas of Latin America where poverty is widespread and was considered a silent and poorly visible disease. It has become a global neglected tropical disease and a significant public health threat worldwide due to globalization over last century. More recently, it has attracted much more attention, and “April 14” has been designated as “World Chagas Disease Day” by the World Health Assembly of 2019 to raise awareness of the disease as a neglected tropical disease, to improve the rates of early treatment and recovery, and to achieve the ambitious goal to interrupt its transmission.